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Effect of depletion of CD34 + cells in myocardial fibrosis. A Schematic showing CD34-CreERT2;Rosa26-tdTomato/DTA mice. Experimental scheme whereby Cre/DTA mice were given tamoxifen for 1 weeks before TAC 0 or TAC surgery. Heart tissue was harvested 4 weeks after TAC surgery. B Representative echocardiography of mice between CD34-CreERT2;Rosa26-tdTomato (Control) and CD34-CreERT2;Rosa26-tdTomato/DTA (Cre/DTA) at 4 weeks after TAC surgery. C Echocardiographic measurement of left ventricle ejection fraction (EF) and fractional shortening (FS) of control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, Cre/DTA group, n = 17; Control group, n = 23. D <t>ELISA</t> detection of the serum ANP and <t>BNP</t> between control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, n = 15 per group. E Representative immunostaining images showing staining of tdTomato and vimentin, DDR2 in control group and Cre/DTA group (Scale bar = 20 μm and 100 μm). n = 10 per group. F Graph showing percentage of tdTomato expression in vimentin + , DDR2 + fibroblast. Data represent mean ± SEM, n = 10
Mouse Bnp Elisa Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of depletion of CD34 + cells in myocardial fibrosis. A Schematic showing CD34-CreERT2;Rosa26-tdTomato/DTA mice. Experimental scheme whereby Cre/DTA mice were given tamoxifen for 1 weeks before TAC 0 or TAC surgery. Heart tissue was harvested 4 weeks after TAC surgery. B Representative echocardiography of mice between CD34-CreERT2;Rosa26-tdTomato (Control) and CD34-CreERT2;Rosa26-tdTomato/DTA (Cre/DTA) at 4 weeks after TAC surgery. C Echocardiographic measurement of left ventricle ejection fraction (EF) and fractional shortening (FS) of control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, Cre/DTA group, n = 17; Control group, n = 23. D <t>ELISA</t> detection of the serum ANP and <t>BNP</t> between control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, n = 15 per group. E Representative immunostaining images showing staining of tdTomato and vimentin, DDR2 in control group and Cre/DTA group (Scale bar = 20 μm and 100 μm). n = 10 per group. F Graph showing percentage of tdTomato expression in vimentin + , DDR2 + fibroblast. Data represent mean ± SEM, n = 10
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Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on <t>BNP</t> and NT-proBNP determined via <t>ELISA</t> assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.
Rat Bnp Elisa Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on <t>BNP</t> and NT-proBNP determined via <t>ELISA</t> assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.
Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on <t>BNP</t> and NT-proBNP determined via <t>ELISA</t> assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.
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Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on <t>BNP</t> and NT-proBNP determined via <t>ELISA</t> assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.
Rat Brain Natriuretic Peptide Bnp Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio csb e07970h human pro atrial natriuretic peptide elisa kit
Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on <t>BNP</t> and NT-proBNP determined via <t>ELISA</t> assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.
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Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on <t>BNP</t> and NT-proBNP determined via <t>ELISA</t> assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.
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Image Search Results


Effect of depletion of CD34 + cells in myocardial fibrosis. A Schematic showing CD34-CreERT2;Rosa26-tdTomato/DTA mice. Experimental scheme whereby Cre/DTA mice were given tamoxifen for 1 weeks before TAC 0 or TAC surgery. Heart tissue was harvested 4 weeks after TAC surgery. B Representative echocardiography of mice between CD34-CreERT2;Rosa26-tdTomato (Control) and CD34-CreERT2;Rosa26-tdTomato/DTA (Cre/DTA) at 4 weeks after TAC surgery. C Echocardiographic measurement of left ventricle ejection fraction (EF) and fractional shortening (FS) of control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, Cre/DTA group, n = 17; Control group, n = 23. D ELISA detection of the serum ANP and BNP between control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, n = 15 per group. E Representative immunostaining images showing staining of tdTomato and vimentin, DDR2 in control group and Cre/DTA group (Scale bar = 20 μm and 100 μm). n = 10 per group. F Graph showing percentage of tdTomato expression in vimentin + , DDR2 + fibroblast. Data represent mean ± SEM, n = 10

Journal: Stem Cell Research & Therapy

Article Title: Single cell and lineage tracing studies reveal the impact of CD34 + cells on myocardial fibrosis during heart failure

doi: 10.1186/s13287-023-03256-0

Figure Lengend Snippet: Effect of depletion of CD34 + cells in myocardial fibrosis. A Schematic showing CD34-CreERT2;Rosa26-tdTomato/DTA mice. Experimental scheme whereby Cre/DTA mice were given tamoxifen for 1 weeks before TAC 0 or TAC surgery. Heart tissue was harvested 4 weeks after TAC surgery. B Representative echocardiography of mice between CD34-CreERT2;Rosa26-tdTomato (Control) and CD34-CreERT2;Rosa26-tdTomato/DTA (Cre/DTA) at 4 weeks after TAC surgery. C Echocardiographic measurement of left ventricle ejection fraction (EF) and fractional shortening (FS) of control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, Cre/DTA group, n = 17; Control group, n = 23. D ELISA detection of the serum ANP and BNP between control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, n = 15 per group. E Representative immunostaining images showing staining of tdTomato and vimentin, DDR2 in control group and Cre/DTA group (Scale bar = 20 μm and 100 μm). n = 10 per group. F Graph showing percentage of tdTomato expression in vimentin + , DDR2 + fibroblast. Data represent mean ± SEM, n = 10

Article Snippet: The levels of ANP and BNP in mouse serum were detected by Mouse ANP ELISA kit (Abcam, ab267800) and Mouse BNP ELISA kit (Novus, NBP2-70,011) according to the protocol provided.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Immunostaining, Staining, Expressing

Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on BNP and NT-proBNP determined via ELISA assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.

Journal: RSC Advances

Article Title: Yixinshu ameliorates hippocampus abnormality induced by heart failureviathe PPARγ signaling pathway

doi: 10.1039/c7ra10650e

Figure Lengend Snippet: Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on BNP and NT-proBNP determined via ELISA assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.

Article Snippet: Commercial ELISA test kits were used in the assessment of the hippocampal samples and serum: rat IL1-beta ELISA kit (eBioscience, USA), rat TNF-alpha ELISA Kit (eBioscience, USA), rat IL-6 ELISA kit (eBioscience, USA), rat BNP ELISA kit (Novus, Hong Kong) and NT-proBNP ELISA kit (Novus, Hong Kong).

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Marker

Fig. 2 YXS protects the hippocampus in rats with heart failure. (A) Expression of the tight junction proteins ZO-1 in the hippocampi of rats. Original Western blots shown on the upper panels. b-Actin was used as a loading control. Bar graphs are gray values. (B) Expression of the proteins AQP4 in the hippocampus of rats. Original Western blots shown on the upper panels. b-Actin was used as a loading control. Bar graphs are gray values. (C–E) The levels of pro-inflammatory cytokines, IL1-beta, TNF-alpha and IL-6, were detected by ELISA. Data are mean SD. vs. model, *p < 0.05, ***<0.001; vs. sham, #P < 0.05. n ¼ 4–6 animals per group.

Journal: RSC Advances

Article Title: Yixinshu ameliorates hippocampus abnormality induced by heart failureviathe PPARγ signaling pathway

doi: 10.1039/c7ra10650e

Figure Lengend Snippet: Fig. 2 YXS protects the hippocampus in rats with heart failure. (A) Expression of the tight junction proteins ZO-1 in the hippocampi of rats. Original Western blots shown on the upper panels. b-Actin was used as a loading control. Bar graphs are gray values. (B) Expression of the proteins AQP4 in the hippocampus of rats. Original Western blots shown on the upper panels. b-Actin was used as a loading control. Bar graphs are gray values. (C–E) The levels of pro-inflammatory cytokines, IL1-beta, TNF-alpha and IL-6, were detected by ELISA. Data are mean SD. vs. model, *p < 0.05, ***<0.001; vs. sham, #P < 0.05. n ¼ 4–6 animals per group.

Article Snippet: Commercial ELISA test kits were used in the assessment of the hippocampal samples and serum: rat IL1-beta ELISA kit (eBioscience, USA), rat TNF-alpha ELISA Kit (eBioscience, USA), rat IL-6 ELISA kit (eBioscience, USA), rat BNP ELISA kit (Novus, Hong Kong) and NT-proBNP ELISA kit (Novus, Hong Kong).

Techniques: Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay