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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: Single cell and lineage tracing studies reveal the impact of CD34 + cells on myocardial fibrosis during heart failure
doi: 10.1186/s13287-023-03256-0
Figure Lengend Snippet: Effect of depletion of CD34 + cells in myocardial fibrosis. A Schematic showing CD34-CreERT2;Rosa26-tdTomato/DTA mice. Experimental scheme whereby Cre/DTA mice were given tamoxifen for 1 weeks before TAC 0 or TAC surgery. Heart tissue was harvested 4 weeks after TAC surgery. B Representative echocardiography of mice between CD34-CreERT2;Rosa26-tdTomato (Control) and CD34-CreERT2;Rosa26-tdTomato/DTA (Cre/DTA) at 4 weeks after TAC surgery. C Echocardiographic measurement of left ventricle ejection fraction (EF) and fractional shortening (FS) of control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, Cre/DTA group, n = 17; Control group, n = 23. D ELISA detection of the serum ANP and BNP between control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, n = 15 per group. E Representative immunostaining images showing staining of tdTomato and vimentin, DDR2 in control group and Cre/DTA group (Scale bar = 20 μm and 100 μm). n = 10 per group. F Graph showing percentage of tdTomato expression in vimentin + , DDR2 + fibroblast. Data represent mean ± SEM, n = 10
Article Snippet: The levels of ANP and BNP in mouse serum were detected by Mouse ANP ELISA kit (Abcam, ab267800) and
Techniques: Control, Enzyme-linked Immunosorbent Assay, Immunostaining, Staining, Expressing
Journal: RSC Advances
Article Title: Yixinshu ameliorates hippocampus abnormality induced by heart failureviathe PPARγ signaling pathway
doi: 10.1039/c7ra10650e
Figure Lengend Snippet: Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on BNP and NT-proBNP determined via ELISA assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.
Article Snippet: Commercial ELISA test kits were used in the assessment of the hippocampal samples and serum: rat IL1-beta ELISA kit (eBioscience, USA), rat TNF-alpha ELISA Kit (eBioscience, USA), rat IL-6 ELISA kit (eBioscience, USA),
Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Marker
Journal: RSC Advances
Article Title: Yixinshu ameliorates hippocampus abnormality induced by heart failureviathe PPARγ signaling pathway
doi: 10.1039/c7ra10650e
Figure Lengend Snippet: Fig. 2 YXS protects the hippocampus in rats with heart failure. (A) Expression of the tight junction proteins ZO-1 in the hippocampi of rats. Original Western blots shown on the upper panels. b-Actin was used as a loading control. Bar graphs are gray values. (B) Expression of the proteins AQP4 in the hippocampus of rats. Original Western blots shown on the upper panels. b-Actin was used as a loading control. Bar graphs are gray values. (C–E) The levels of pro-inflammatory cytokines, IL1-beta, TNF-alpha and IL-6, were detected by ELISA. Data are mean SD. vs. model, *p < 0.05, ***<0.001; vs. sham, #P < 0.05. n ¼ 4–6 animals per group.
Article Snippet: Commercial ELISA test kits were used in the assessment of the hippocampal samples and serum: rat IL1-beta ELISA kit (eBioscience, USA), rat TNF-alpha ELISA Kit (eBioscience, USA), rat IL-6 ELISA kit (eBioscience, USA),
Techniques: Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay